Abstract: Adipose-Derived Stromal Cells Demonstrate Superiority over Bone Marrow-Derived Stromal Cells in Bone Healing by Enhancing Vasculogenesis in a Murine Model of Irradiated Mandibular Fracture Injury

RESULTS: Inhibition of the BMPR signaling pathway inhibited Runx2 and BSPII gene expression of primary human mesenchymal stem cells (hMSCs) on MC-GAG. In contrast, inhibition of the MEK/ERK axis downregulated BSPII expression on Col-GAG independent of Runx2 expression. While inhibition of the BMPR signaling pathway resulted in decreased mineralization on both Col-GAG and MC-GAG, inhibition of the MEK/ERK axis only affected mineralization on Col-GAG. When the mechanistic detais were evaluated in greater detail, inhibition of the BMPR pathway reduced both Smad1/5 phosphorylation and Runx2 protein expression on both MC-GAG and Col-GAG. Inhibition of the MEK/ERK axis downregulated phosphorylation of ERK1/2 and JNK1/2 without affecting Smad1/5 phosphorylation or Runx2 expression.